Recombinant human PAM was expressed as a C-terminally truncated protein lacking its transmembrane and cytosolic domains to facilitate its secretion.
Submit a review and receive a US/€18/£15/CAN amazon gift card if you include an image - US/€7/£6/CAN Amazon card for reviews without an image.
Peptidylglycines with a neutral amino acid residue in the penultimate position are the best substrates for the enzyme.
Involved in the final step of biosynthesis of alpha-melanotropin and related biologically active peptides.
The discovery of PAM in cilia, cellular antennae that sense and respond to environmental stimuli, suggests that much remains to be learned about this ancient protein.
Homozygous mutation of this gene results in embryonic lethality during fetal growth and development, edema, abnormal yolk sac vasculature, thin arterial walls, and abnormal bronchial epithelial morphology.
conversion of the peptidyl-alpha-hydroxyglycine derivative into an alpha-amidated product at physiological p H by peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PHL) at alkaline p H spontaneous conversion; EC 184.108.40.206 is often called peptidylglycine alpha-amidating monooxygenase (PAM) and the alpha-amidated product is mentioned as the product of the reaction, but the alpha-amidation of glycine-extended peptides is a two-step process catalyzed by 2 enzymes: 1.
A critical role for peptide C-terminal amidation was apparent when the first bioactive peptides were identified.
PAM is required for the biosynthesis of peptides such as Substance P, neuropeptide Y, oxytocin, vasopressin, and calcitonin (3).
The structures of both catalytic cores have been determined, revealing how they interact with metals, molecular oxygen, and substrate to catalyze both reactions.
Although not essential for activity, the intrinsically disordered cytosolic domain is essential for PAM trafficking.
Limited to verified customers in USA, Canada and Europe.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.